Browsing by Author "Harman, James G."
Now showing items 1-2 of 2
-
CRP subunit association and hinge conformation changes in response to cAMP binding: Analysis of C-helix cysteine-substituted CRP
Tomlinson, Steven R.; Tutar, Yusuf; Harman, James G. (AMER CHEMICAL SOC, 2006)We investigated the characteristics of 13 CRP variants having cysteine substituted at positions 113, 115, 116, 117, 118, 120, 122, 124, 126, 127, 129, 130, or 131, positions that span the length of the CRP C alpha-helix. ... -
Effect of salt bridge on transcription activation of CRP-dependent lactose operon in Escherichia coli
Tutar, Yusuf; Harman, James G. (ELSEVIER SCIENCE INC, 2006)Expression of catabolite-sensitive operons in Escherichia coli is cAMP-dependent and mediated through the CRP:cAMP complex binding to specific sequences in DNA. Five specific ionic or polar interactions occur in cAMP binding ...